Test Procedure

The WSU MDL is an open lab system allowing the flexibility to use multiple reagents and testing protocols. Currently, MDL is using the following Molecular genetic active virus testing protocols using RT-PCR.

Thermo Fisher Scientific, Inc. TaqPath COVID-19 Combo Kit

The Thermo Fisher TaqPath™ “Gold Standard” first utilizes a nucleic acid extraction step and then evaluates three different sequence targets of the viral genome.  It currently requires a nasopharyngeal, oropharyngeal, or anterior nares swabbing placed in Viral Transport Media.  Specificity is essentially 100% and sensitivity is essentially 100% as well.

Yale School of Public Health, Department of Epidemiology of Microbial Diseases, SalivaDirect 
SalivaDirect is non-extraction method and evaluates a single nucleic acid sequence target on the Coronavirus genome.  This requires one ml of saliva in a sterile tube provide to healthcare professionals by the MDL.  Specificity is essentially 100% and sensitivity approximately 94.6%.

Thermo Fisher Scientific TaqPath ™

  • FDA EUA approved for use on all CDC approved respiratory specimens (such as nasopharyngeal, oropharyngeal, nasal, and mid-turbinate swabs, nasopharyngeal aspirate and bronchoaveolar lavage (BAL) specimens)
  • The gold standard for testing: full viral RNA extraction on the KingFisher'" Flex with real-time reverse transcription polymerase chain reaction (RT-PCR) on the QuantStudio 7
  • Coming soon: Multiplex Assay: allows for detection of
    SARS-CoV-2, Influenza A, Influenza B, and RSV from
    one analyzed specimen
  • Coming soon: Addition of approved saliva specimen for infection detection.
  • Targets 3 different regions of the viral genome
  • Testing Specificity: 100% 
  • Testing Sensitivity: 100% 
  • FDA Emergency Use Authorization
  • Healthcare Provider Fact Sheet (US)
  • Patient Fact Sheet (US)

Yale SalivaDirect™

  • FDA EUA approved for saliva specimens collected in sterile cups or tubes without addition of any preservatives providing greater ease in sample collection.

  • Extraction-free RT-PCR: Proteinase K heat treatment followed by real time reverse transcription polymerase chain reaction (RT-PCR) on the Applied Biosystems 7500 Fast Dx
  • Utilizes combinations of alternate and substitute reagents, at each step adding redundancy and flexibility in the supply chain.

  • Enables non-invasive, frequent sampling and reduces the need for trained healthcare professionals during collection
  • Targets 1 region of the viral genome

  • Testing Specificity: 100%
  • Testing Sensitivity: 94.6% 
  • FDA Emergency Use Authorization
  • Healthcare Provider Fact Sheet (US)
  • Patient Fact Sheet (US)
   

MDL RSV/Influenza/COVID-19 (RIC) Assay

The MDL RIC test utilizes the "gold standard" for viral PCR testing. First, nucleic acid extraction is performed followed by RT-PCR that looks for multiple viruses in the sample. This test requires nasopharyngeal, oropharyngeal, or anterior nares swab placed in viral transport media. Specificity is essentially 100% and sensitivity is >97%.

  • Approved for use on all CDC approved upper respiratory specimens (such as nasopharyngeal, oropharyngeal, and mid-turbinate swabs)
  • The gold standard for testing: full viral RNA extraction on the KingFisher™ Flex with real-time reverse transcription polymerase chain reaction (RT-PCR) on the Applied Biosystems™ 7500 Fast Dx
  • Coming soon: Addition of approved saliva specimen for infection detection
  • Targets 3 different viruses
  • Targets multiple regions of the COVID-19 viral genome
  • Testing Specificity: 100%
  • Testing Sensitivity: >97%
  • Patient Information Sheet
  • Healthcare Provider Information Sheet
  • Partner Information Sheet


Visit WSU MDL

Laboratory Hours:
Monday-Friday 6:00 AM - 6:00 PM
Saturday 8:00 AM to 11:00 AM
Saturdays only for partner drop-off

Holiday Closures:
Labor Day – Closed
Veterans Day: Open Regular Hours
Thanksgiving Day: Closed
Day after Thanksgiving:
Open 8:00am -11:00am
Saturday after Thanksgiving:
8:00am - 11:00am
Christmas Eve: 6:00am – 2:00pm
Christmas Day: Closed
New Year’s Eve: 6:00am – 2:00pm
New Year’s Day: Closed

4174 S. Oliver, Building 174H, Wichita, KS 67210

Customer Service Support

Customer Service Hours:
Monday-Friday 8:00 AM - 5:00 PM

Phone: (316) 978-8600
Fax: (316) 978-8610
Email: mdl@wichita.edu


Specimen Collection Hours

Monday-Friday 6:00 AM - 6:00 PM

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