Alert

Effective April 15th, MDL will transition from providing 24 hour result turnaround to 48 hour result turnaround. If you have any questions, please email us at mdlsupport@wichita.edu or call at (316) 978-8600.

MDL Testing Menu & Collection Guides

Complete List of Testing Information Sheets & Guides

Access all testing information sheets and collection guides in one PDF.

Microbiology Testing

 

Bacterial Identification – Aerobic

Sample Source

  • Pure culture isolate on suitable agar (solid) medium; slant or plate

Collection Container

  • Suitable agar (solid) medium for isolate submitted. Can be slant or sealed plate

Test Method and Description

  • Identification methods may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. Genus and species are reported on aerobic bacterial isolates whenever possible.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 2 – 4 days

Additional Information 

Bacterial Identification – Anaerobic

Sample Source

  • Pure culture isolate on suitable agar (solid) medium; slant or plate

Collection Container

  • Suitable agar (solid) medium for isolate submitted. Submit a sealed plate in an anaerobic transport bag to maintain viability.

Test Method and Description

  • Identification methods may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. Genus and species are reported on aerobic bacterial isolates whenever possible.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 2 – 4 days

Additional Information 

Bacterial Identification and Susceptibility – Aerobic 

Sample Source

  • Pure culture isolate on suitable agar (solid) medium; slant or plate

Collection Container

  • Suitable agar (solid) medium for isolate submitted. It can be slant or sealed plate.

Test Method and Description

  • Identification methods may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. Genus and species are reported on aerobic bacterial isolates whenever possible.
  • Susceptibility testing may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disc diffusion.
  • NOTE: Susceptibility testing is performed depending on source and identification. If the source and identification do not warrant susceptibility testing per CLSI guidelines, MDL will notify the client piror to continuing with testing.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 2 – 4 days

Additional Information 

Bacterial Identification and Susceptibility – Anaerobic

Sample Source

  • Pure culture isolate on suitable agar (solid) medium; slant or plate in an anaerobic environment.

Collection Container

  • Suitable agar (solid) medium for isolate submitted. Submit a sealed plate in an anaerobic transport bag to maintain viability.

Test Method and Description

  • Identification methods may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. Genus and species are reported on anaerobic bacterial isolates whenever possible.
  • Susceptibility testing will be minimal inhibitory concentration (MIC) (gradient strip diffusion).
  • NOTE: Susceptibility testing is performed depending on source and identification. If the source and identification do not warrant susceptibility testing per CLSI guidelines, MDL will notify the client prior to continuing with testing.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 3 – 5 days

Additional Information 

Blood Culture

Sample Source

  • Blood (specify collection site)

Collection Container

  • FA Plus Bottle

  • FN Plus Bottle
  • PF plus Bottle

Test Method and Description

  • Culture using automated continuous monitoring system.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 5 – 7 days

Additional Information 

Blood Culture (Positive – Referred) Identification

Sample Source

  • Positive blood culture bottle

Collection Container

  • Blood culture bottle(s) that have been flagged positive by the client’s instrumentation
  • Culture on suitable agar (solid) medium plate
  • NOTE: For clients that are able, plates should be subbed and submitted with the bottle(s) to expedite results

Test Method and Description

  • Identification methods may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. Genus and species are reported on anaerobic bacterial isolates whenever possible.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 1 – 4 days

Additional Information 

Blood Culture (Positive – Referred) Identification and Suspectibility

Sample Source

  • Positive blood culture bottle

Collection Container

  • Blood culture bottle(s) that have been flagged positive by the client’s instrumentation.
  • Culture on a suitable agar (solid) medium plate
  • NOTE: For clients that are able; plates should be subbed and submitted with the bottle(s) to expediate results.

Test Method and Description

  • Identification methods may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. Genus and species are reported on anaerobic bacterial isolates whenever possible.
  • Susceptibility testing may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disc diffusion. 

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 1 – 4 days

Additional Information 

Body Fluid Culture – Aerobic, Anaerobic, Gram Stain

Sample Source

  • Synovial
  • Peritoneal
  • Pericardial
  • Pleural
  • Other (indicate specific source)

Collection Container

  • Sterile Container

Test Method and Description

  • Conventional aerobic and anaerobic bacterial culture techniques with selective and non-selective media.  
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels. 
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion. 

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 4 – 5 days

Additional Information 

Cerebral Spinal Fluid Culture and Gram Stain – Lumbar

Sample Source

  • Cerebral Spinal Fluid (specific source/collection method: i.e.: lumbar puncture, shunt, ventricular shunt, etc.)

Collection Container

  • Sterile Container

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.   
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.  
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.  

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 4 – 5 days

Additional Information 

Cerebral Spinal Fluid Culture – Shunt – Aerobic, Anaerobic, Gram Stain

Sample Source

  • Cerebral Spinal Fluid (specify source/collection method: i.e.: lumbar puncture, shunt, ventricular shunt, etc.)

Collection Container

  • Sterile Container

Test Method and Description

  • Conventional aerobic and anaerobic bacterial culture technique with non-selective media.   
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.  
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.  

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 4 – 5 days

Additional Information 

Genital Culture

Sample Source

  • Genital

  • Vaginal
  • Abscess
  • Endocervical
  • Rectovaginal
  • Urethral/Penial
  • Other

Collection Container

  • ESwab
  • ESwab Minitip Flocked Collection Kit (Urogential)

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 3 – 4 days

Additional Information 

Group A Screen by Culture

Sample Source

  • Throat

Collection Container

  • ESwab

Test Method and Description

  • Conventional aerobic bacterial culture technique, screening for presence or absence only of Group A (Streptococcus pyogenes).
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 1 – 3 days

Additional Information 

Group B Screen by Culture

Sample Source

  • Rectovaginal

Collection Container

  • ESwab

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 3 – 4 days

Additional Information 

Lower Respiratory Culture with Gram Stain

Sample Source

  • Sputum

  • Tracheal Aspirate
  • Bronchial Wash
  • Bronchial Alveolar Laveage (BAL)
  • Biopsy

Collection Container

  • Sterile Container
  • Sterile Saline Tube 

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 3 – 4 days

Additional Information 

MRSA Screen by Culture

Sample Source

  • Anterior Nares

Collection Container

  • ESwab

Test Method and Description

  • A nasal swab is inoculated onto Spectra MRSA agar, a selective and differential chromogenic medium used in qualitative detection of nasal colonization of methicillin-resistant Staphylococcus aureus (MRSA).

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 24 – 32 hours

Additional Information 

Neisseria Gonorrheae Culture 

Sample Source

  • Vaginal

  • Endocervical
  • Urethral/Penial
  • Urethral Discharge
  • Vaginal Discharge
  • Genital
  • Lesion
  • Throat

Collection Container

  • ESwab
  • ESwab Minitip Flocked Collection Kit (Urogential)

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 3 – 4 days

Additional Information 

Tissue Culture – Aerobic, Anaerobic, Gram Stain

Sample Source

  • Tissue (specify site)

Collection Container

  • Sterile Container

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 4 – 5 days

Additional Information 

Upper Respiratory Culture

Sample Source

  • Throat Swab
  • Oropharyngeal Swab
  • Nasal Swab
  • Nasopharynx Swab

Collection Container

  • ESwab

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday - Sunday

Turnaround Time

  • 3 - 4 days

Additional Information 

Urine Culture

Sample Source

  • Urine - specify collection method (i.e., clean catch, foley catheter, cystoscopy, etc.)

Collection Container

  • BD Vacutainer Urine C&S Tube
  • Unpreserved Sterile Container

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday - Sunday

Turnaround Time

  • 2 - 3 days

Additional Information 

Wound Culture – Aerobic, Gram Stain

Sample Source

  • Wound (specify source/site)

Collection Container

  • ESwab
  • Sterile Container

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 4 – 5 days

Additional Information 

Wound Culture – Aerobic, Anaerobic, Gram Stain

Sample Source

  • Wound (specify source/site)

Collection Container

  • ESwab
  • Sterile Container

Test Method and Description

  • Conventional aerobic bacterial culture technique with selective and non-selective media.
  • Identification methods (when appropriate) may include any of the following: conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and commercial identification panels.
  • Susceptibility testing (when appropriate) may include minimal inhibitory concentration (MIC) (broth microdilution or gradient strip diffusion) or disk diffusion.

Testing Schedule

  • Daily; Monday – Sunday

Turnaround Time

  • 4 – 5 days

Additional Information 

Molecular Tests

RSV/Influenza/COVID–19 PCR (RIC)

Sample Source

  • Nasopharyngeal
  • Orophayrngeal

Collection Container

  • Viral Transport Media (M4 or M5) with flocked Nasopharyngeal or Oropharyngeal Swab

Test Method and Description

  • Real-time reverse transcription polymerase chain reaction (RT-PCR) test intended for qualitative detection of nucleic acid for Respiratory Syncytial Virus (RSV) A/B, Influenza Virus A/B, SARS-CoV-2 Virus.

Testing Schedule

  • Daily; Monday - Friday at 11:30 AM

Turnaround Time

  • < 48 hours

Additional Information 

SalivaDirect™ COVID–19 PCR (COVSAL)

Sample Source

  • Saliva

Collection Container

  • 5mL Screw Cap Eppendorf Tube

Test Method and Description

  • Real-time reverse transcription polymerase chain reaction (RT-PCR) test intended for qualitative detection of nucleic acid from SARS-CoV-2.

Testing Schedule

  • Daily; Monday - Friday at 11:30 AM

Turnaround Time

  • < 48 hours

Additional Information

Visit WSU MDL

Community Collection Partner Drop-Off Hours

Monday - Thursday: 7:00 AM - 6:00 PM
Friday: 7:00 AM - 2:00 PM
Saturday - Sunday: Closed
4174 S. Oliver, Building 174H, Wichita, KS 67210

COVID-19 Testing Available By Appointment

Monday-Thursday: 7:00 AM - 4:00 PM
Friday: 7:00 AM - 2:00 PM
Saturday-Sunday: Closed
4174 S. Oliver, Building 174H, Wichita, KS 67210

Holiday Closures

Thanksgiving:
    Thurs., November 28, 2024
    Fri., November 29, 2024

Christmas:
    Wed., December 25, 2024

New Years Day:
    Wed., January 1, 2025

Customer Service Support

Customer Service Hours:
Monday - Friday:
8:00 AM - 12:00 PM
1:00 PM - 5:00 PM

Phone: (316) 978-8600
Fax: (316) 978-8610
Email: mdlsupport@wichita.edu

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